Sentence examples for overnight rotation from inspiring English sources

After overnight rotation at 4°C, protein A agarose beads were added incubated at 4°C for an additional hour.

Cell lysates were then incubated by overnight rotation at 4°C with 4 μg of either normal rabbit IgG or primary antibody, followed by rotation-incubation with protein A-agarose for 2 h.

Samples were dissolved at a concentration of 4 mg/ml in DMSO-LiBr by overnight rotation at room temperature, followed by 30 min heating in an oven at 80 °C obtaining clear sample solutions.

After a 4°C overnight rotation, the suspension was centrifuged as above resulting in a magnesium insoluble DNA-pellet (P2) and magnesium soluble-supernatant (S2) fraction (Pellet in Figure  1C).

BMMA infiltration was carried out with decreasing concentrations of ethanol:BMMA (2 1,1 1,1 2) before overnight rotation in 100% BMMA and polymerization under UV light at 4°C for 10hrs in gelatin BEEM capsules.

Immunoprecipitates were collected on Protein G agarose beads by overnight rotation, washed four times with lysis buffer, re-suspended in 2× Laemmli sample buffer, and were subjected to SDS-PAGE followed by Western blot analysis.

After being rinsed, stained, and dehydrated, samples were then infiltrated with a 1 1 mix of acetone and Epon 812 (EMS cat#14120) overnight with rotation.

Equal quantities of cell lysates (200 µg) were incubated (4°C) with VEGFR2 antibody (2 µg) overnight, with rotation.

The corresponding antibody was added to the cleared cell extract and incubated overnight with rotation at 4°C.

The remaining supernatant (90 µl) was then incubated with 15 µl of anti-FLAG M2 affinity gel (Sigma) at 4°C overnight with rotation.

4µL purified anti-CaRF antibody (#4510)[15] was added to each lysate (WT and KO) and incubated overnight with rotation at 4°C.