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Before and after treatment, each animal was fasted overnight, measured for plasma insulin levels and challenged i.p. with 2 g/kg D-glucose (Sigma-Aldrich, St . Louis MO; intraperitoneal glucose tolerance test, IPGTT) followed by serial sampling of blood glucose.
Glucose variability overnight measured as standard deviation was similar during the two interventions.
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The final national rating will probably be lower because the overnight measures only 48 major markets.
The mice were then left until 0900 h the following morning when the amount of pellets remaining was again recorded to give the overnight measure.
For continuous variables, repeated-measures regression models were used to test the differences between the two treatment arms, accounting for correlated data from the same participant and for the overnight measures, adjusting for the bedtime blood glucose value.
Each rating point in the Milwaukee market, which is among the overnight markets measured by Nielsen, equals 9,011 viewers.
Saturday's 42nd-season kickoff averaged a 5.8 rating in the overnight markets measured by Nielsen.
On the day of the experiment, the body weights of the rats, which were fasted overnight, were measured, and the rats were then anesthetized with pentobarbital sodium (60 mg/kg body weight, intraperitoneally).
Similarly, pellet containing biomass was washed three times with distilled water and dried overnight to measure the cell dry weight.
Immediately after electrophoresis, the gels were fixed in 40% methanol and stained with a Coomassie Blue Solution (Brillant Blue G and methanol used at a ratio of 1∶4 respectively) overnight for measuring the amount of proteins.
The cartilage samples were freeze-dried overnight after measuring the wet weight.
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