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Overnight lays of Drosophila embryos were used for Figure 4.
Embryos from the Dichaete-Dam, SoxN-Dam and Dam-only transgenic lines in each species were collected after overnight lays and DNA was isolated using minor modifications to the protocol of Vogel and colleagues [ 96].
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Some 14 hours later, the full extent of the political earthquake that unfolded overnight was laid bare.
The overnight shift was laid off first.
The motion was laid overnight and could not be changed after News Corp abandoned the bid.
We take a long, blustery walk along Traigh Ear, where an overnight storm has laid out seaweed like a natural history exhibit, then over the headland to Traigh Iar, where we earmark the in-between rocks for a future seal swim.
In 2010, smoke was tumbling out of the station, but it turned out that it was only a cigarette that had not been quenched, and had been laying overnight in a flower basket.
Embryos were laid overnight at 23°C on apple juice agar dishes, dechorinated in bleach and mounted on Greiner Lumox gas-permeable culture dishes (Sigma) in halocarbon oil 700 (Sigma) or on glass slides with double-sided tape with Voltalef oil (VWR International).
To obtain oviposition preference indexes (OI), the number of eggs on each half of the plate was counted after females laid eggs overnight: OI = (no. of eggs laid on experimental food − no. of eggs laid on control food)/total no. of eggs laid).
In the overnight dispatch, Mr. Plouffe lays out the argument – rooted in math and politics – for how he believes the door is closing on Senator Hillary Rodham Clinton's chance to capture the Democratic nomination.
Crosses were performed in laying cages with apple juice agar plates and flies left to lay overnight at 22°C with embryos collected the following day, with the exception of the Src42ADN and draper RNAi overexpression experiments, in which embryos were laid at 29°C overnight.
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