The sand and surf on south Santa Monica bay appeared virtually free of oil after an overnight clean-up, but officials weren't sure if more tar would show up.
As the police recovered the bodies, county and municipal crews worked overnight to clean up spilled fuel and other debris.
The charcoal Celite mix was first preconditioned in 100%% acetonitrile overnight to clean and activate the charcoal mixture.
Soak the beans overnight in clean water.
Wipe up as much as you can with a dry cloth, let it dry overnight, then clean as described above.[7].
Occasionally, completely disassemble and wash the pipe in rubbing alcohol and let soak overnight, then clean with q-tips and toilet paper.
The coverslips were then washed with 2 N HCl overnight and cleaned with DI water.
5 µg of pB-SK zTH (kindly provided by W. Driever) were linearized with XhoI at 37°C overnight and cleaned up using phenol/chloroform. 1 µg of purified DNA was subjected to Digoxigenin-RNA synthesis following the manufacturer's instruction (DIG RNA Labeling Kit, Roche) and T3 RNA polymerase (Fermentas, St. Leon-Rot, Germany).
All the glassware were soaked in 5% HCl overnight and cleaned with deionised water before use.
DNA was linearized with NotI at 37°C for overnight and cleaned up by DNA Clean/Extraction Kit (GeneMark Inc., Taiwan).
If you want you may even buy light and regular pads (Always thin, Always slender, Always clean), overnight pads (Stayfree overnight), pantyliners (Always pantyliner, Always wrapped pantyliners), and if you feel that it is necessary, get some feminine wipes (Playtex petals).
