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Moreover, cardiomyocytes were degenerated and the expression of Cav1.2 protein was increased in iron overload group as compared to control.
Control group received the vehicle, iron overload group received ferrous sulfate intraperitoneally (IP) for 4 weeks, iron overload + verapamil received ferrous sulfate and verapamil IP concurrently for 4 weeks and iron overload + vitamin D group received ferrous sulfate IP and vitamin D3 orally concurrently for 4 weeks.
The only other major diagnostic category with a difference of more than five percentage points between the groups was digestive-system-related with more frequent reporting in the non-fluid-overload group (18%) compared with the fluid overload group (13%).
In addition, the patients were all classified in the correct iron overload group by the tested machines.
The serum ferritin levels of the current hemochromatosis/iron overload group ranged between 350 to 1900 μg/L (tested in a pathology lab).
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The control and iron overload groups (6 plates each) received 200 μl of 0.1 M ammonium citrate (Sigma) or 200 μl of 0.1 M ferric ammonium citrate (Sigma, iron content of 16.5-18.5%) per well, respectively.
Rats in the Al-overload group received chronic intragastric administration of aluminum gluconate (Al3+ 200 mg/kg per day), followed by administration of 0.5% CMC-Na 2 h later.
Throughout the 20 weeks of experiments, a total of nine rats died, including two rats in control group, three rats in Al-overload group, two rats in BPS-6 group and two rats in BPS-12 group.
The 75 rats were randomly divided into five group depending on body weight (each n = 15): a control group, an aluminum-treated group (Al-overload group), and three BPS-treated groups (6, 12, and 24 μg⋅kg-1 BPS, marked as BPS-6 group, BPS-12 group and BPS-24 group, respectively).
Compared to the control group, at the stage of spatial learning function test from d 3 to d 4, the time for the Al-overload group to learn to navigate the maze significantly increased (p < 0.05); on d 5 at the stage of spatial memory function test, the exploring time was significantly longer, p < 0.01.
A comparison of hospital demographics between the fluid overload and non-fluid-overload groups is shown in Table 2.
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