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These data, and structural overlays (data not shown), indicate that the structures are similar irrespective of the crystalline environment in which they have been determined and it is therefore only necessary to describe a generic TtrD structure.
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The overall sequence identity shared between TtrD with these two proteins is only 17 and 21%, respectively, and structural overlays primarily align the four-helix bundles (data not shown).
We have investigated if a similar inhibition mechanism is present in EAG1, however, no binding of N- and C-terminus was detected on an overlay blot both in the presence and absence of Ca2+/CaM (data not shown).
Only HUH-REISO cells, pre-cultured by agarose overlay, showed enough plasticity to form endothelial like tubes within 4h (data not shown).
The 1H-15N correlation peaks of full-length SNF were quite similar to the overlay of those from isolated RRM1 (residues 1 104) and RRM2 (residues 134 216) (data not shown), indicating that each RRM is relatively independent and there lacks inter-domain interaction.
We used a neutral phylogenetic tree to overlay the average frequency of derived alleles between Angus and Holstein cattle (data not shown).
Overlaying the release curves after the initial 4 days showed no significant differences in the release kinetics (data not shown).
Conducting an agar overlay experiment, we revealed an inhibited growth of Lactococcus lactis, resulting in a zone of clearing around BAA-2069 (data not shown).
Overlay of the two images confirmed that the avidin signal corresponds to the lower molecular weight mature Kar2 band (data not shown).
p.i (data not shown).
(detailed data not shown).
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