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The laminin overlay experiment also detected a 225 kDa protein of endogenous laminin expressed by DG-deficient cells as well as wildtype cells.
In the light of the lipid overlay experiment, this observation supports the idea that AdcA binding to PI(3 P contributes to its endosomal recruitment.
Conducting an agar overlay experiment, we revealed an inhibited growth of Lactococcus lactis, resulting in a zone of clearing around BAA-2069 (data not shown).
In particular, the fluorescent gp120 overlay experiment of the type used in Figure 2 could be attempted with human or primate LN sections.
This result differs from previous work in which an I-CaM overlay experiment was performed with purified rat huntingtin and I-CaM failed to bind huntingtin, suggesting that huntingtin and CaM did not bind directly (3).
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We repeated the same overlay experiments as shown in Fig. 4, but this time, we kept the accelerating voltage fixed at 3.0 keV and adjusted the negative-bias voltage to produce a final landing energy between 3.0 and 1.0 keV.
An eye tracker was used to mark fixated areas by either removing a semiopaque gray overlay (Experiments 1 and 4) as portions of the display were fixated or by adding the overlay once the eye left a segment of the image (Experiments 2 and 4).
Blot overlay experiments were performed according to [41].
To identify the receptor(s) of PfRH5 on human erythrocytes, we used gel overlay experiments in which erythrocyte ghost proteins, separated on SDS-PAGE, were incubated with rRH5 (Fig. 6, rRH5) or with Dd2 culture supernatant (Fig. 6, Dd2).
Even though the lipid organization and the composition of this new phase is not yet fully understood, it is well conceivable that the packing parameter of Gb3 might influence this lipid compaction and reorganization process, which was corroborated by fluorescence overlay experiments.
In the latter case, bacterial overlay experiments were carried out to locate the protein of interest.
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