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In fact, a comprehensive study has shown that sequences with at least 25% identity over at least 80 residues have backbone structures that overlay closely.
The structural superposition shows that the β2GC subunit of the homodimer and the βGC subunit of the heterodimer overlay closely, while the β1GC subunit of the homodimer and the αGC subunit of the heterodimer present distinct orientations.
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In support of this assignment, the Trp fluorescence melt curve overlays closely with that from 230 nm observed in CD (Figure 3D).
R166 overlays closely in these structures.
The Vpu ELV motif overlays closely with a canonical acidic dileucine-sorting motif when bound to AP2.
Indeed, the EFRET populations of the unbound and bound Nup153FGPxFG also overlay very closely with respect to τ, which indicates similarly fast dynamics of both forms (Kalinin et al., 2010).
The structures of the E190A and K137A variants differ very little from that of wild-type caspase-3, where one observes that the interfaces and the active sites overlay very closely with those of wild-type caspase-3.
In the far UV-CD region, the spectrum of refolded CHMO showed two minima at 205 nm and 215 nm and it was observed to overlay very closely with that of native CHMO (Fig. 4B), suggesting that the refolded CHMO has a very comparable secondary structure to the soluble and native CHMO.
In the interface, the six conserved water molecules are present in the mutant, and the interface overlays very closely with that of wild-type caspase-3.
Superposition of the energy minimized structure of decoquinate overlays more closely with the positioning of famaxodone and MOAS in co-crystallographic studies.
During the helix fluctuation, the side chain of Y195 rotates approximately 90°, although the hydroxyl retains its orientation toward helix 3. The structure of the T140F variant shows that the dimer interface overlays very closely with that of wild-type caspase-3, and five of the six conserved water molecules are retained in this variant.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com