The distribution patterns of various types of erosion were identified by GIS overlay of buffer strips and soil erosion maps.
Using the tools of view-shed analysis, spatial overlay analysis, buffer and other methods on the GIS platform, eco-tourists' perception degree is calculated.
The resuspended pellet was overlayed on buffer S2 (0.35 M sucrose and 0.5 mM MgCl2), followed by a centrifugation (1,500 g for 5 minutes at 4°C).
Following centrifugation for 10 minutes at 12,000× g, lysates were overlaid onto a 10 50% (w/v) sucrose gradient prepared by overlaying 10% buffered sucrose (20 mM Tris [pH 8.0], 140 mM KCl, 5 mM MgCl2, 0.5 mM DTT, 0.1 mg/ml cycloheximide) onto 50% buffered sucrose followed by horizontal diffusion for 3 hours.
750 mL reservoir medium was used for experiments devoted to conductance and heating, producing a 2 mm buffer overlay physically uniting the anodic and cathodic reservoirs.
Untreated slides were overlayed in RNase buffer and also incubated at 37°C for 8 hrs.
Strips were equilibrated in SDS-equilibration buffers I and II (with DTT and iodoacetamide, respectively) and placed onto an SDS-PAGE gel [ 26] using an overlay of SDS-gel buffer solidified with agarose (0.5%).
The suspension was transferred into a rotor tube, overlayed with 2 ml buffer B and centrifuged (SW41, 70 900×g, 70 min).
The 25,000× g membrane pellet, which contained the highest activity, was suspended in 0.75 ml 1.25 M sucrose buffer and overlayed with 0.5 ml 1.1 M and 0.5 ml 0.25 M sucrose buffer (Golgi isolation kit; Sigma).
Proteins were translated using a cell-free bilayer system [26], where the translation reaction is separated from translational substrate buffer by carefully overlaying in a 6-well multi-well plate.
