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Briefly, each pair of primers contained a non-overlapping sequence at the 3′-terminus and a primer-primer complementary (overlapping) sequence at the 5′-terminus.
Overlapping sequence files between two 1-Mb files were inputted manually when necessary.
Each peptide was 14 15 amino acids long and contained 5 amino-acid overlapping sequence with the adjacent peptide.
We counted overlapping sequence tags at 50 bp resolution.
Overlapping sequence fragments were assembled using Geneious version 5.5.7 created by Biomatters (available from http://www.geneious.com).
Sequences in the primer regions were corrected using overlapping sequence alignment.
aContigs are defined as more than one read joined by overlapping sequence.
However, the lack of sufficient overlapping sequence homologies prevented us from performing phylogenetic analyses.
From these, we generated draft shotgun sequences that provided large contigs with 26 kb of overlapping sequence.
However, the user can find the Spacing length instead of the Overlapping length and Spacer sequence instead of Overlapping sequence.
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Overlapping ICs were then concatenated into larger non-overlapping sequence blocks, called Identical Sequence Tracks (ISTs).
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