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In doing so, we could prevent false positives and false negatives on the feature overlapping scenarios found in our study.
Sense/antisense gene pairs were defined based on three types of overlapping scenarios: (1) annotated in Ensembl annotation; (2) just like the first type, except that the overlap comes from assembled contigs that went beyond Ensembl annotation; (3) splicing-based overlap supported by novel junction reads.
Several potentially overlapping scenarios of early-stage Alzheimer pathogenesis have been discussed, with an emphasis on the protective effects of AT-1 mediated antihypertensive response on cytoskeleton remodeling, along with neuronal activation of oncogenes, luteinizing hormone signaling and insulin-related growth regulation, forming a pleiotropic model of its early stages.
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As a specific type of coexistence, a partial overlapping scenario is assumed.
Consequently, we estimated the parameters under the Overlapping scenario and SOS selection model.
For all loci, the Overlapping scenario is the most likely (>52%% relative probability under LOS and 38-85 % under NFDS, respectively) and the Southern route scenario significantly the less likely (<1 % under both LOS and NFDS).
Indeed, under both alternative selection models and for all loci, the Overlapping scenario is the most likely and the Southern route scenario is (almost always significantly) the less supported (see Additional file 4: Table S4 and Additional file 5: Table S5).
We consider the no overlap scenario even though by definition the reader-reader collision problem occurs only with overlapped tags.
These include: advantage of overlapping in different scenarios, special properties of optimal overlap, and impact of parameter values on overlapping decisions.
A relevant aspect to take into account in this respect is that in some scenarios overlapping communities might be more meaningful.
By overlapping the expected scenarios with the VGI provided by different types of observers directly in the field (Cross-Validation Module), the decision maker is able to update the hazard scenario and to verify the number and location of the exposed elements in order to plan a possible evacuation.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com