However, I-FISH scoring was more likely to be influenced by fading of signals, overlapping of cells, and angle of tissue slicing from paraffin blocks, which could result in interobserver variability.
The sections reveal LCs to be typically rounded and well dispersed throughout the epidermis, with no noticeable overlapping of cells (Figure 5B,C).
The deficiency of tangential tension as a result of the disappearance of the marginal bundle prevents contact paralysis and leads to overlapping of contacting cells.
In Arabidopsis, at least five PIN genes are expressed in different or overlapping groups of cells in roots, maintaining an auxin sink and maximum in the root tip [35], [36], generating a lateral auxin gradient in root cap columella cells upon gravi-stimulation [37], and controlling basipetal auxin transport in root distal and elongation zones [38] [42].
Even the recordings of granule cell EPSCs and spikes were done for different (though perhaps overlapping) populations of cells, so we cannot say how the EPSCs relate to the specific firing patterns.
However, conventional MR imaging cannot accurately evaluate this invasive behavior due to overlapping of the edema and tumor cells.
After dual staining of 3-D HT-29 cells with the M-cell marker (sialyl Lewis A) and enterocyte marker (syndecan-1) antibodies, we observed very little overlap of cells expressing both markers, demonstrating two different cellular populations within the aggregates (Figure S6) [55], [60], [61], [61].
Similar colocalisation of GLP-1 and PYY was found in the ascending colon in humans with 69% overlap of cells labelled with each individually.
In this study, we seek to capture the qualitative behaviour of these materials, which is primarily to prevent the separation and overlap of cells and facilitate force transmission between cells.
Overlap of cells labeled in red and cells labeled in green was not observed, and since the Nk×2.1-BACcre line labels MGE-derived cortical interneurons, the MGE can thus be discounted as a source of 5HT3aR interneurons [ 25].
At confluence, a tightly packed monolayer of slightly elongated cells with some overlapping of adjacent cells was evident.
