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After the application of all these restrictions commented on above, we eventually had 715 co-directional overlaps with overlapping lengths longer than 60 bps, which were examined manually.
Scripts implemented in Perl language were performed to extract and analyse the spacers and the overlaps between adjacent genes and all the information related (spacing & overlapping lengths, spacing & overlapping sequences, gene orientations, phases, protein functions, gene COGS and stop & start codons of the genes).
The SEDs of unit cells, tablet and matrix are presented for different overlapping lengths and inclination angles, from which maximum SEDs and optimum overlapping lengths are determined.
Given that a specific standardized window is overlapped by n LDhat-windows with (overlapping) lengths L1, L2,.., Ln, and estimated recombination rates R1, R2,.., Rn, the average recombination rate of the standardized window was calculated as <img src="http://journals.plos.org/plosone/article/asset?id=info?doi/10.1371/journal.pone.0008650.e003.PNG" class= inline-graphic"/> for i = inline-graphic
Since the models may be of different, even non overlapping, lengths we normalized the RMSD on the number of the aligned residues according to simple scaling proposed by Carugo and Pongor [ 32]: R M S D 100 = R M S D 1 + 1 2 log (N 100 ) where RMSD100 is the estimated RMSD when 100 residues are aligned and N is the number of aligned residues.
When suitable primer pairs in the pre-set overlap length range were unavailable, the overlapping lengths of some adjacent PCR products were raised automatically.
Similar(47)
The lap shear strength is not directly proportional to overlap length, adherend thickness, adherend width and scarf angle for the brittle adhesive studied in the paper.
However, the user can find the Spacing length instead of the Overlapping length and Spacer sequence instead of Overlapping sequence.
Tested overlap lengths were in the range of 4 100 mm.
Different failure modes are observed in different overlap lengths.
Also shown are the experimental results for other overlap lengths.
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