Sentence examples for overlapping cdna from inspiring English sources
Ks values between overlapping cDNA sequences were calculated using the Ka/Ks calculator (Zhang et al. 2006) software package, using both aligned cDNA sequences and their aligned, translated amino acid sequences as input.
For the LAMC2 mutation analysis we sequenced overlapping cDNA fragments spanning the entire open reading frame in each two affected and non-affected sheep.
However, in brief, three gene-specific overlapping cDNA per sample were generated from extracted RNA and subjected to 40 cycles of PCR.
Overlapping cDNA sequences in HJEB-affected sheep were amplified using primer pairs from exon 17 to intron 18 and primer pairs from intron 18 to exon 20.
Three gene-specific overlapping cDNA per sample were generated and subjected to 40 cycles of PCR with FastStart HiFi polymerase (Roche, Indianapolis, IN) to make eight partly overlapping amplicons that cover the entire PR and the first 236 codons of RT genes.
Samples of the virus harvest were also used to generate RNA samples as described above and used in RT-PCR assays using appropriate primer sets to obtain overlapping cDNA fragments corresponding either to selected regions of the genome or, in some cases, to the near complete genome (lacking the extreme 5' untranslated region on the 5' side of the poly(C) tract).
So we observe far fewer conserved blocks overlapping cDNAs than would be expected (81 * 1465/2805= 42.3) if all conserved blocks were parts of cDNAs.
EST sequences with homology to repat genes were used to design specific (as well as nested) primers for amplification and cloning of overlapping 5'and 3' cDNA fragments, which were sequenced and assembled into complete mRNA sequences.
Support for expression at the locus provided by overlapping EST, cDNA or microarray probeset.
The set of 25 genes overlapping between cDNA microarrays [ 13] and SAGE were not statistical significant (p > 0.05).
Moreover, two overlapping RBP4 cDNA fragments spanning from exon 2 to 6 and covering the complete coding sequence (CDS) were amplified from Tai-Zumu individuals.
