Sentence examples for overlapping and extension from inspiring English sources

These three sequences have been extended by electronic cloning (EST overlapping and extension) and 5′race and have been deposited in GenBank.

During this overlap and extension selection procedure, care was taken that the extension BACs were of the same haplotype as the clones in the tiling path, by verifying that their overlapping BES had a 100% nucleotide match to the tiling path sequence.

The loci were regarded as identical if the locus regions after extension overlapped and were mapped in the same direction.

The Pex secretory sequence (ss) [39] was fused to the TNT.R3 receptor [5] using overlapping extension PCR and the resulting ssTNT.R3 gene cloned into pCB302-3.

All possible combinations of heavy and light chains, among all immunoglobulin isotypes, were included by using a mixture of primers and overlapping extension PCR.

Dynamic error tolerance and overlapping extension exploration: ABMapper can give more chance to reads that would be extended exceeding a cutoff, to find out whether it could be mapped in full length by allowing one additional error.

To clarify how AP-2γ regulates the gene expression of ErbB2, CDH2, HPSE and IGSF11 through binding to the predicted sites, wild-type and mutant of AP-2γ-ChIP fragment luciferase vectors were generated through appropriate restriction enzyme digestion and overlapping extension PCR.

Similarity to double fusion is that outermost primers with other PCR components are mixed at the same time, and overlap extension reaction and full length fused DNA amplification reaction are working in one tube.

A C425S site-directed mutant was constructed by overlapping extension PCR [40], and was then expressed, and purified using the same protocol.

Previously published methods for E. coli [25] and Salmonella Enteritidis [13] use overlapping extension PCRs and/or cloning to generate TCs and rely on co-electroporation of the TC together with an I-SceI expressing plasmid.

Primers (Table 1) corresponding to both strands with mutated bases in the middle were designed to produce mutant R1-15, R3-36 and R4-28 by overlapping extension PCR [ 24].