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Only those cells in which TOM20/LAMP1 overlapped (yellow fluorescence) in up to five intracytoplasmic areas were considered positive to mitophagy in our analysis.
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Cells with overlapping yellow and red labels represent what is called a memory trace.
Simultaneously, they expressed MT (Figure 5E) evidenced by overlapping yellow stain in merged micrograph (Figure 5F).
Inversion of the region repositions the two probes within close proximity to each other, visualized as overlapping yellow signals on metaphase chromosomes (Fig. 3A).
B) Overlapping yellow-dotted spheres delineating the space to be filled by hydrophobic packing in the main pocket of the binding site.
To quantify the degree of co-localization [25], we analyzed the areas where NG2 (red (R)) and GFAP (green (G)) pixels overlapped became yellow (Y) using the built-in co-localization tool of MetaMorph software (version 7.5.2.0; Molecular Devices).
The cells that expressed DPFP-BG20_A01 and those that contain EGP-BG97/192_B06 mRNAs were largely overlapped, demonstrating yellow signal.
g Overlap (yellow) between AUC60 (red) and SPECT (green) within the tumor boundary (dotted line).
Overlap (yellow) between SPECT peptide uptake signal (green) and DCE-MRI AUC60 data (red) is shown in Fig. 2g.
Interestingly, in ΔY-derived DCs, Giantin and surface Kb demonstrated a significant (p = 0.0004) degree of overlap (yellow) and some triple colocalization (white) (p = 0.007) was also apparent (Figures 5C and 5F).
Arrows indicated overlap (yellow) of CD163+ cells (green) and DC-SIGN+ cells (red).
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