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All overlapping domains examined show a compact N-terminal coiled-coil intorting into a partially opened C-terminal partner, where the opposing coiled-coils at the overlap junction face each other at up to ∼90° twist angles.
Structural variants were identified using de novo assembly-based approach employing ABySS and Trans-ABySS [ 13] and the alignment-based SV detection tool Minimum Overlap Junction Optimizer (MOJO) (https://github.com/cband/MOJO).
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The perfectly aligned array solves this problem because there are "absolutely no tube-tube overlap junctions," Rogers says.
In this paper, we fabricate a novel ZnO nanowires overlapping junction (ZnO NWS-OLJ) electrode via a facile and cheap solution chemical routine.
Open image in new window Fig. 1 a An overlapping junction.
Overlapping junctions were merged.
In another variation, the selected hexamers are ligated into defined strings by complimentary hexamers that overlap the junction in the opposite strand of a double helix [ 14].
Whereas the percentage of junctions in genes expressed at low levels was negligible, for highly expressed genes up to 70% of the exons were overlapped by junction reads (Fig. 5D).
Primer pairs were chosen to overlap exon/intron junctions to prevent amplification of genomic DNA (Supplementary Table 2).
For BRCA1 and BRCA2 expression analysis, probes and primers were designed so that they overlapped splice junction, thereby avoiding the potential amplification of genomic DNA.
In addition, in in vitro cultures of primary ECs, VE-cadherin shows a particularly broad reticular pattern at sites of cellular overlap (reticular junctions).
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