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prolonged overlap extension PCR.
Overlap extension PCR.
splicing by overlap extension PCR.
Overlap extension of the three fragments was conducted as described in Protein Domain Library Generation by Overlap Extension (PDLGO) (Gratz and Jose, 2008).
The new method is called protein domain library generation by overlap extension (PDLGO).
Overlap extension PCR is another simple technique for constructing recombinant DNA but is not commonly used.
Herein we present a simple, universal, efficient gene synthesis method based on sequential overlap extension polymerase chain reactions (OE-PCRs).
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In brief, the S89G mutation was introduced in exon 6 using an overlap extension-PCR method.
Both PCR products were combined with single-overlap extension PCR.
Mutants were constructed using the single-overlap extension procedure.
DNA-fusion constructs were generated by overlap-extension PCR.
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