PSCFP2-Mito and eGFP-mito were made by using Age1 and Xba1 to remove dsRed cDNA from dsRED2-mito (Clontech), and ligating in PSCFP2 with the same restriction endonuclease overhanging ends from PSCFP2-C1 from Evrogen (Moscow, Russia) or eGFP-C1 (Clontech/Invitrogen).
Some habitat complexity is provided by aquatic vegetation, and cover is sometimes available from overhanging vegetation or from overhanging banks.
She ripped off the horizontal wireless masts on Furiouss starboard side, carried away five overhanging lifeboats from the port side of, and just missed the third and fourth ships in line.
In cities such as London it is done to prevent branches from tangling with overhead wires and from overhanging streets and to promote growth of a denser foliage.
They're sometimes called monkey fish for their ability to coil their body and spring from the water to snatch insects from overhanging branches.
Finally, she emphasizes the group's goals with simple examples, like opposing moves to lower water-quality standards for the benefit of the operators of animal feedlots, or opposing the removal of protective designations from scenic roads where the moss still hangs from overhanging oak branches.
Fronds from overhanging trees weave a green canopy against the sun.
And as we climbed up higher on the ridgeline, I nibbled on tart strawberry guavas plucked from overhanging trees.
From overhanging limestone cliffs, which rise above 600 ft (180 m) along its seaward (western) edge, it descends to sand dunes along the east.
Gay, with a partner of 17 years, he has ruddy cheeks, a quick smile and a plane of brown hair overhanging his brow, from which he must regularly wipe away sweat during intense discussions.
