Sentence examples for overhang type from inspiring English sources

Because Cas9 nickases can generate either overhang type while Cas9 nucleases produce blunt ends, the enzyme can be adapted to the needs of the cell type and organism.

Telomerase-deficient survivors typically show clear alterations in telomere structure type I survivors undergo dramatic amplification of Y′ elements and retain short TG overhangs, whereas type II survivors show modest amplification of Y′ elements but amplify their TG overhangs (Teng and Zakian, 1999).

Different design scenarios related to overhang design, construction type, wall insulation level and orientation are tested using uncertainty analysis to reveal their respective influence for designing a naturally ventilated building with consistent performance.

The TaqLinker contains a 2-nt 5'-overhang (in bold type) complementary to the TaqαI -digested biotinylated DNA.

The effect of changes in the length and type of ssDNA overhangs, such as a short overhang versus a long overhang, a 3′-overhang versus a 5′-overhang, and one versus two overhangs, on Pif1-promoted strand annealing was investigated.

A How you design the buildings influences energy usage and the way you orient a building — where you put windows, the type of overhangs, and whether you use daylight versus artificial light.

Therefore, 256 different overhangs can be created using a type IIs restriction endonuclease that produces a 4 nt overhang.

The full-length human PDCD4 3′-UTR (1917 bp) was PCR-amplified from cDNA with the following primers: 5′-ccg ctcgagATATAAGAACTCTTGCAGTCT-3′ and 5′-ataagaat gcggccgcACAGAGGATCTTTACATGTTTA-3′ containing NotI and XhoI restriction site overhangs, respectively (indicated in italic type).

(A ) Cleavage of a 5' stem-loop overhang substrate (15 nM) by wild type, α1-deleted, and OB mutant Dna2 in the presence of 15 nM Rpa.

To take advantage of these rules, flat-bodied hulls were developed with long overhangs and light displacement; the resulting skimming-dish type was exemplified in the defender of the America's Cup of 1903, the Reliance, which had overhangs totaling more than 50 feet (15 m) on a waterline length of about 90 feet (27 m).

In construction of the initial library, the adapter containing the sequencing primer hybridization site (Sol-L) was modified before its NN overhang to incorporate the recognition motif of the Type IIS restriction enzyme GsuI (5'-CTGGAG-3').