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The algorithm evaluates each possible transformation for several design variables in each floor plan, such as floor plan orientation and reflection, window orientation and size, overhang size, fin size, and wall translation.
In general, the junctions with short overhang size and lower coverage were considered as false positives, which are often caused by non-specific or error alignment.
To minimize the false positive rate, we required that the overhang size had to be greater than 20 bp with at least two reads spanning the junctions.
After filtering the splice junctions by two criteria (for details, see Materials and Methods) - an overhang size of more than 20 bp and at least two reads to support the splice junctions - a junction data set of 397,321 confident junctions that we believe to be true splice junctions was obtained.
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The alignments of the raw RNA-seq reads to the random junctions revealed that 99.9% of false positive junctions had overhang sizes with fewer than 20 bp.
In sharp contrast, the alignments of the annotated junctions indicated that most (98.6%) of annotated junctions had larger overhang sizes (Additional file 24A).
To explore the capabilities of the developed method, it is tested on a typical room model; and the impacts of the design variables, including building orientation, window size, overhang system, and the glazing and walls specifications on the building energy demands are studied in four main weather conditions of Iran.
The EC performance was compared to a state-of-the-art spectrally selective low-e window with the same divided window wall, window size, and overhang as the EC configuration.
To evaluate the capability and effectiveness of the approach, the developed method is applied to a single room model, and the effect of building architectural parameters including, the building orientation, the shading overhang specifications, the window size, and the glazing and the wall material properties on the building energy consumption are studied in four major climatic regions of Iran.
Without limiting the length of the overhangs and gap sizes to 2 Mbp, the increase in N50 scaffold sizes in the Budgerigar_v6.3 is 17.1 Mbp (which we think could be an artifact).
In this work, CFD-based numerical simulations are employed to investigate the effects of overhangs of various sizes on the wind-driven rain wetting of a low-rise building under various wind and rain conditions.
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