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Read alignments with mapq scores of 0 and/or a splice junction overhang of less than 6 bp were then filtered out.
Similar(57)
Sequences that formed a single stem-loop with 0 to 3 nt overhangs at both ends and with a MFE of less than -25 [ 13] were retained for further filtering.
Thus, sgRNA pairs with an offset distance of less than −30 bp should leave a 5′ overhang.
When there is excess capacity and an overhang of debt, interest-rate cuts tend to be less effective in reviving demand.
In fact, at a national level lower wages would almost certainly lead to fewer jobs -- because they would leave working Americans even less able to cope with the overhang of debt left behind by the housing bubble, an overhang that is at the heart of our economic problem.
In nominal terms, the average home is now worth 16% less than at the peak in 2006, and the large overhang of unsold houses suggests that prices have further to fall.
It is not immediately obvious why countries should have become dumber or less creative over the past decade, so there may be other reasons – the overhang of debt or a malfunctioning financial sector – that explain the slowdown.
Tate & Lyle admitted that it had forced to swallow less lucrative deals from customers buying its Splenda sweetener -- blaming a "significant overhang of unsold Chinese sucralose" that had flooded the market.
Trim any overhang of pastry.
The bumpy overhang of his brow.
But the overhang there is an overhang of personal debt on working people that is substantial.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com