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Single-cell WGA and WTA techniques currently being used in the research setting have technological limitations, and an important challenge to implementing SCS in the clinic is overcoming errors that may be introduced by amplifying the minute amount of DNA or RNA in a single cell and properly validating the sequencing results.
Not just any effort, but persistent, intensive effort that comes with focusing on overcoming errors by pushing oneself to the edge of one's knowledge and skills.
In addition, it is suggested validation in a larger number of samples for overcoming errors from multiple testing.
In contexts like the one in which DREAM is active, where people of different nationalities (and languages) find themselves working together, this has turned out to be an essential instrument for overcoming errors and misunderstandings caused by the language used.
In overcoming errors associated with PCR amplification, ultra deep sequencing, read filtering, read alignment, and the accurate detection of single nucleotide polymorphisms (SNP's), haplotype reconstruction does not only provide insight into within-host viral evolution, but also epidemiological insight into dissecting possible transmission events [ 18].
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The researchers involved in the writer's block study became convinced that "allowing for error" was integral to overcoming writing block.
Although NGS technology appears to have an important role in future forensic studies, more work is required to fully achieve this goal, which includes overcoming problems with low-template library preparation, error rate, type estimations and issues with NGS data processing and mining.
Executive control involves planning, decision making, error detection, giving novel responses, or overcoming habitual actions [ 1].
Internal forward models are used, for example, to estimate motor errors during performance and to adjust motor commands online overcoming feedback delays, or to plan motor behavior in advance by testing several alternatives and estimating their outcomes before the execution of the motor behavior.
The executive control network includes the monitoring and resolution of conflict between computations in different neural areas, such as planning or decision making, error detection, conditions judged to be difficult or dangerous, regulation of thoughts and feelings, and the overcoming of habitual actions (Raz and Buhle 2006).
Overcoming barriers is about freedom.
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