To overcome the specificity problem, a comprehensive footprinting approach was formulated to identify TF-specific operator motifs and was applied to the LacI-family of TFs in the model gram positive organism, Lactobacillus plantarum WCFS1.
To overcome that specificity, we used SLAC and FEL [ 92] for detecting positive selection while allowing variation in synonymous rate.
Potential solutions to overcome the specificity problem of chemical proteomics are (i) to include unrelated drugs or the matrix itself as negative controls, (ii) to identify and subtract from the analysis the abundant proteins or core proteome, and (iii) to perform a competitive pull-down to rank the most relevant targets [ 28].
There is increasing interest in multi-allele vaccines to overcome strain-specificity against polymorphic vaccine targets such as Apical Membrane Antigen 1 (AMA1).
As it overcomes 'context specificity' [ 1, 16] through a wide sampling process, this selection instrument is considered more reliable than the Single-Station Personal Interview (SSPI), no matter how structured the latter may be [ 17, 18].
Cell sorting can also be used as an initial step in sample preparation to enrich for specific cell types in a cell mixture to overcome sensitivity and specificity limitations of arrays.
Dual time point PET imaging has been proposed as alternative method to overcome the low specificity of SUVmax in the differentiation of benign from malignant lesions, including thyroid incidentalomas (Lee et al. 2014).
Furthermore, the use of a mixture of defined epitopes could lead to an effective broad range immune response which has the potential to overcome both strain specificity of the pathogen and the MHC restriction of the host.
Saccharin (SAC) based compounds may provide an avenue to overcome CA isoform specificity, as they display both nanomolar affinity and preferential binding, for CA IX compared to CA II (>50-fold for SAC and >1000-fold >1000-folds conjugated to a carbohydrate moiety).
The aim is to improve the accuracy of current approaches and in particular to overcome the low specificity.
