Similar results were obtained with different growth media or using a strategy of co-expression with chaperones; conditions that usually are effective to overcome the expression of insoluble proteins from actinobacteria [27].
Additionally, the disadvantage of C2-dependent growth of pyruvate decarboxylase-deficient (Pdc−) S. cerevisiae was overcome by expression of the Candida tropicalis PDC1 gene.
Thus, restoration of only the 79 bp immediately downstream of the PolyA addition site is insufficient to overcome variegated expression.
Having overcome the expression limitations of nPTB we were now able to compare its splicing repressor activity with PTB.
To overcome this, expression levels were normalized against vector genome copy number.
Interestingly, all four cell lines that acquired resistance to ZSTK474 overexpressed IGF1R, and their resistance was overcome by expression knockdown of IGF1R.
If the failure of target gene activation is responsible for the low reprogramming efficiency in the prolonged cultures, this should be overcome by expression of these targets.
Indeed, they overcome limited expression of specific functions when microbial populations evolve for instance under conditions of lactose limitation in Escherichia coli[ 1, 4], glucose limitation in yeast [ 5, 6], or antibiotic pressure in Salmonella[ 2, 3].
In another report, senescence induced by B-RAFV600E or N-RASQ61R did not depend on p16INK4a or p53 but could be partially overcome by expression of the oncogenic transcription factor c-MYC [ 36].
Mutations of a foetal or neonatal muscle isoform would be overcome on expression of the mature isoform, as seen for example, in the rare congenital myasthenic Escobar syndrome (Hoffmann et al., 2006).
