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However, the E2F3-transduced line continued to grow at the same rate 24 hours after reaching confluency (Figure 2 C, filled circles), suggesting that forced expression of E2F3 can overcome contact inhibition.
We analysed anchorage-independent growth in a soft agar assay (Missale et al, 1998), where cells proliferate without cell or surface contacts and must overcome contact inhibition.
Malignant cell transformation is characterized by the ability of cells to overcome contact inhibition and to grow in a semisolid environment.
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We observed that TSPO potentiates proliferation, motility and transmigration capabilities as well as the ability to overcome contact-induced cell growth inhibition of glioma cells.
In the longer term, when material problems are sufficiently under control, we believe the future lies in the development of heterostructures and inserted interface layers to overcome contacting problems and quantum heterostructures and superlattices to facilitate low-noise readout.
However, only the class III isoform efficiently induced colony formation in NSLT and was capable of overcoming contact inhibition to allow cell proliferation at high densities (Figure 4C and 4D).
Of note is the marked decrease of proliferation under confluent and over-confluent conditions, implying a role of MIF in overcoming contact inhibition.
Of note was the marked decrease of proliferation under confluent and over-confluent conditions, implying a role of MIF in overcoming contact inhibition.
Our study provides new insight into the role of MIF in tumor growth and progression by showing the importance of MIF for overcoming contact inhibition.
Our study adds another puzzle stone to the role of MIF in tumor growth and progression by showing the importance of MIF for overcoming contact inhibition.
Use of soil mixing techniques for reagent delivery provides a means to overcome contaminant/reagent contact limitations.
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