Furthermore, the strong survival ability of the Père David's deer has allowed these animals to repeatedly overcome bottlenecks when building new breeding populations.
A thorough understanding of the factors that determine expression levels and strategies to overcome bottlenecks are important for predictable protein production.
Since productively infected mosquitoes are capable of transmitting DENV for life, and also take multiple bloodmeals over a single gonatrophic cycle [34], an increased ability of DENV to overcome bottlenecks at the midgut infection stage could have implications for virus transmission.
The simplicity and accessibility of SPMA can overcome bottlenecks due to difficult sample preparation and handling in urinary proteomics.
This approach has previously been shown to overcome bottlenecks in engineering of heterologous pathways and to improve sugar utilization in S. cerevisiae engineered for pentose fermentation [ 22, 23].
Our results demonstrate a powerful strategy for applying systems biology methods to rapidly identify metabolic engineering targets and overcome bottlenecks in performance of engineered strains.
Such successes will provide an incentive to overcome bottlenecks in hPSC technology such as improving cell maturity and industrial scalability whilst reducing cost.
Providers are assumed to have, or must be given and supported in using, the autonomy to be able to respond in a creative way to overcome bottlenecks in attaining results at their level.
Maternal and perinatal mortality audit applies to two of the five objectives in the Every Newborn Action Plan but data on successful approaches to overcome bottlenecks to scaling up audit are lacking.
Attempts to overcome bottlenecks in the TAG synthesis pathway, including overexpression of the enzymes acetyl-CoA carboxylase (ACCase) and the acyl-transferase step, 1,2-diacylgycerol acyltransferase (DGAT), did not yield enhanced TAG contents [ 27, 28].
We strongly believe that the strategy used in this paper can be applied to resolve many other unclear genotype phenotype correlations and so help to overcome bottlenecks in further improvement of strains.
