Two commercially obtained paired-read datasets were combined, and an automatic gap-closure algorithm was applied, to overcome encountered assembly problems related to repetitive DNA.
This suggests that at these sites, the RSC proteins are needed to overcome the assembly of nucleosomes by the ISWI complex in order to activate the gene.
Given that several isoforms were only found in one species, greater sequencing depth is required for our analysis to overcome incomplete assemblies and sampling biases, previously observed in genomic sequences of Pseudomonas syringae strains [ 47].
The proposed connection overcomes the assembly problems due to the non-standardized bamboo geometry.
Thus, coilin's essential function in embryos is to promote macromolecular assembly of snRNPs, likely by concentrating snRNP components in CBs to overcome rate-limiting assembly steps (Strzelecka et al. 2010b).
To overcome these difficulties, assembly was performed by extension of each contig separately against all reads to create overlaps and to find sequence spanning contigs.
College is seen as the pinnacle of social life because the condensed geography, flexible schedules, similar demographics and outgoing energy enable people to overcome the barriers to assembly.
However, in differentiating ciliated cells, this finely tuned mechanism controlling the one-mother-one-daughter centriole ratio is overcome to allow the assembly of the masses of centrioles that become basal bodies.
The morphogenesis checkpoint thereby works to delay mitotic entry until a critical bud size is reached, and defects in the actin cytoskeleton or septin assembly are overcome.
Moreover, using the recently developed Trinity software, any disadvantages related to short reads can be overcome, resulting in the assembly of transcriptome results without a reference genome being as reliable as those with an available reference genome [ 29].
