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The overall protocol time was less than three hours.
Increasing the degree of automation by combining some or all of the 4 steps (cell lysis, hybridisation, sample treatment, CE analysis) would make the overall protocol time even shorter and frequent at-line monitoring more practical.
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Simplification of the overall protocol might greatly eliminate the possibility of contamination during sample processing, while shortening assay time.
The overall protocol was also approved by the IRB from the New York Blood Center (Protocol 415-05).
The overall protocol adherence was 94%.
The overall protocol layout is visualized in Figure 1.
Overall protocol duration was 7 18 hours (6 30, 7 55).
JMPF designed the overall protocol and supervised the project.
The overall protocol does not require ethical review.
The overall protocols are similar to those used in [ 6].
Hinshaw et al. [ 34] showed in an animal experiment that the triple-freeze protocol produces a zone of necrosis that is essentially identical to the double-freeze protocol despite a shorter overall freeze time (15 versus 20 min).
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