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These measurements will provide an assessment of the overall motility.
Together, these observations support a model of motility in which regions of the cell edge exhibit one of a limited number of motility modules that, together, determine the overall motility function.
ER-mitochondria MCSs have been shown to play a role in mitochondrial fusion-fission and overall motility.
Overall motility and viability were both very high in control and viral exposed spermatozoa (respectively: 80.0% and 79.2% in control semen; 80.0% and 69.9% in exposed semen).
Kymographs were generated to quantify overall motility, defined as the percentage of mitochondria that moved within a 6-minute time-lapse movie.
In addition, serum components acting as chemoattractants further stimulated cell migration of EGFP MTA1-expressing PANC-1 cEGFP MTA1-expressingt the expression of MTA1 accelerates the overall motility of PANC-1 cellsuggesting
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Modulation of filament bending and twisting dynamics has been linked to regulatory actin-binding protein function, filament assembly and fragmentation, and overall cell motility.
Interestingly, application of glutamate (1mM), which serves as an agonist on both ionotropic and metabotropic glutamate receptors, did not result in either significant changes on any of the 3 dendritic morphological parameters or on overall process motility (data not shown).
In addition, Sucnr1−/− microglia showed overall compromised motility, which likely contributes to their trapping.
When Oa1−/− melanocytes were subjected to similar analysis, an overall lower motility of melanosomes was observed in untreated conditions.
Although the authors claim an overall reduced motility of mitochondria, contrasting to our findings, they specifically find retrograde transport events to be affected, which is in line with our observations.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com