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E. bieneusi genes were mapped to homologues in the E. cuniculi genome to determine the overall level of conservation between these two species.
Comparisons of VP3 amino acid sequences from the fifty four BTV isolates revealed an extremely high overall level of conservation, with polymorphic positions representing only 7.77% of the amino acid sites.
To control for conservation, we carried out the same analysis after stratifying sites by overall level of conservation.
It shows a low overall level of conservation including a striking number of sometimes quite large insertions (fig 4B).
However, the overall level of conservation of U12 introns between plants and animals, and hence the depth of the evolutionary conservation of U12 introns is not known.
The overall level of conservation at the single nucleotide level could be estimated by PhastCons [ 19] based on multiple sequence alignments (MSA).
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For instance, Huang et al[ 7] investigated whether human disease genes differ significantly from their rodent orthologs with respect to their overall levels of conservation and their rates of evolutionary change.
Another observation is that though some assemblers may demonstrate similar overall level of preservation of the 248 CEGs, they differ at the individual conservation group percentages of CEGs (see Figures 2, 3 and Tables S1-S5; Additional file 1).
We estimated the overall level of synteny conservation throughout poxvirus genomes by considering the conservation of neighboring genes between orthologs in pox.
We did this by measuring conservation between human and mouse orthologues, as a proxy for the overall level of evolutionary conservation.
This paper presents a methodology for the synthesis of inter-plant heat-integrated water allocation network (IHIWAN) in industrial parks, to promote the overall level of resource conservation by exploiting the cooperative utilization among multiple plants.
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