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The quantification by densitometry from 3 to 4 independent experiments showed that BIG2 was depleted to 86% and BIG1 to 75% across the overall cell population (Fig. 1B).
Because very few cells exhibit multipolar chromosome segregation, the observed rates of cytokinesis failure represent a very rare event in the overall cell population.
A significant fold change of one gene can be diluted when occurring in a cell type representing only a fraction of the overall cell population being studied.
As infected cells are undergoing latent and lytic replication at the same time and are in the overall cell population, it would be difficult to measure the precise number of cells in the lytic or latent phase of infection and the cells expressing LMP-1 during the early stages of infection.
Alternatively, nucleofection gave a repair efficiency of up to 0.02% of the overall cell population.
Since Stat1 causes reduced cell proliferation, these cells may be enriched in the overall cell population after multiple passages.
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For anticancer drug therapy, it is critical to kill those cells with highest tumorigenic potential, even when they comprise a relatively small fraction of the overall tumor cell population.
Two of the shRNA lentiviruses (TRCN0000097987 and TRCN0000097989; abbreviated as Lenti 87 and Lenti 89 elsewhere in the text) induced significant reductions in Tim expression in the overall ES cell population.
To examine whether the observed inhibition of proliferation of the overall cancer cell population by Rac1 knockdown is due to a specific effect on CSCs, we next tested the growth properties of isolated SP and non-SP cells before and after transduction of Rac1-specific shRNA.
Overall apoptotic cell population by deguelin was increased from 14.7% to 51.1% in a time-dependent manner.
Hence, the subpopulation of ABCG2+ cells within the overall TamR cell population is responsible for the more invasive phenotype of the TamR cells.
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