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Absolute cell counts were calculated from the manually counted overall cell number in a sample and the population frequency assessed by flow cytometry.
Furthermore, inhibition of caveolin-1 expression using a caveolin-1 siRNA significantly attenuated E2-induced up-regulation of proto-oncogenes, cell cycle regulatory proteins, [3H]-thymidine incorporation, overall cell number, and percent of the cell population in S phase, while mediating a concomitant increase in the G0/G1 population.
hCdc14A- and hCdc14B-specific siRNAs caused a drop in the overall cell number (data not shown), suggesting that both isoforms are required for proper cell proliferation.
Furthermore, the number of neurons assessed by anti-MAP2 staining as well as overall cell number per field of view including glial cells assessed by DAPI staining was not changed compared to control cells.
As the overall cell number increased 100 200 fold, and the proportion of competent EPC (CD34+ to CD36+) rose from <0.5% to >50%, the in vitro culture procedure generated the EPC at an efficiency of >10 000-fold.
Although the overall cell number was the same in the cultures the reactivity against the EBV peptide was higher in the cultures with IDO5-specific T cells (figure 6d).
Similar(40)
Overall cell numbers were determined using a commercially available kit (CytoTox 96 non-radioactive Cytotoxicity Assay, Promega) that quantitatively measures lactate dehydrogenase (LDH) release upon cell lysis [ 33].
Interestingly, ddPCR shows that the period of phage proliferation corresponded to an increase in overall cell numbers by a factor of 10 over ∼5 days.
Correspondingly FACS analysis revealed BrdU incorporation within CD133+ retinal cells when incubated with FGF/N2, supporting the hypothesis that increases in overall cell numbers in cultures arise from newly generated cells.
The combination of the two stimuli triggered a significant increase in BAL neutrophils and in overall cell numbers compared to normal controls and led to increased cell differential variability between animals (Table 2).
Such objective analysis is likely to provide greater discriminatory power than is provided by human observations, so that lower overall cell numbers (and probably lower MCM-2 thresholds) would be required, thereby increasing sample adequacy.
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