Sentence examples for overall alignment rate from inspiring English sources

Sequencing performance QC (Step 3) is performed during the alignment process, by estimating specific statistical metrics (that is total number of reads, % of unpaired reads, % of reads aligned 0 times, % reads aligned exactly once, % of reads aligned more than once, and overall alignment rate) for each sequenced sample.

The overall alignment rate of reads to the reference sequence was 97.6% with an average read depth of 12.0× (8.77× to 14.77×).

Since, our read length was ∼100 bp and bowtie 1 has limitations in aligning longer reads, each read was broken down into two separate reads of 45 bp each to increase the overall alignment rate.

Mapping all RNA-seq reads against both transcriptomes (CLC or Velvet/Oases) revealed that the overall mapping success rate, as a measure for assembly quality, was significantly lower for the Velvet/Oases assembly compared to the CLC assembly (see Additional file 1), with an average overall alignment rate of 91.7 % and 73.4 % for the CLC and Velvet/Oases assembly, respectively.

Wilcoxon rank-sum tests were conducted to assess whether or not the initial LIIs and overall alignment rates differed between the test and control groups.

Given these vastly different overall alignment rates and patterns, we chose UMD3.1 to serve as our reference sequence build for assembling the optical map.

The alignment across all 18 samples produced a mean Overall Read Alignment Rate of 96.4 ± 0.4 % and a mean Concordant Pair Alignment Rate of 88.0 ± 0.2 %, confirming that the read alignment was excellent and that data was of high quality.

Overall read alignment rates were above 90%% for all libraries.

The overall mapping rate ranged from 58.3 to 62.2 % and the concordant alignment rate from 51 to 54.7 %.

The authors stated that the severity of the preoperative deformity affected overall alignment postoperatively.

Alignments were then manually adjusted to improve the overall alignment.