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The specific cell growth rate remained at a constant value of 0.014 h−1 over the batches (Table 7; the definitions of the abbreviations used in Table 7 can be found in Table 1).
The succinic acid production rate increased from 0.022 g h−1 to 0.053 g h−1 while that of acetic acid (from 0.012 g h−1 to 0.007 g h−1) and propionic acid (from 0.137 g h−1 to 0.109 g h−1) decreased over the batches.
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However, the ARA contents and ARA yield (42.8% and 10.0 g/L, respectively) were clearly enhanced over the batch fermentation.
An attractive mode of operation is to run the industrial process isothermally and to use a mixture ("cocktail") of different initiators, which is able to spread the polymerization rate over the batch time.
Overall, perfusion mode titers were higher by almost one order of magnitude over the batch culture mode of production.
Blank samples were used to correct for background, and in-house developed algorithms were applied using the pooled QC samples to compensate for shifts in the sensitivity of the mass spectrometer over the batch.
Give air popped popcorn extra flavor by spraying it with non-stick butter spray and sprinkle light salt over the batch.
This total cost needs to be contextualized in terms of its time value, and therefore an appropriate discount rate is applied and an NPV calculation is made over all the batches.
In the second phase, six methylation assays representing observations on the ten assays were measured in the middle-aged and old Dutch twins, who were randomly divided over the measurement batches, and in the follow-up Danish twins, who were all allocated to a single measurement batch.
However, airlines will not have to hand over the first batches of emissions permits until the spring of 2013 to compensate for flights made in 2012.
The system is due to take effect next year, and the airlines would not have to hand over the first batches of permits until the spring of 2013 to compensate for flights made in 2012.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com