Exact(2)
This is consistent with the notion that Taq enzyme is still in excess over template in the diluted Taq reaction after 27 cycles (1.8×109 templates and 8×109 Taq molecules).
The amount of genomic DNA was reduced to a minimum in order to maintain an excess of aqueous droplets over template molecules.
Similar(57)
Soon afterward, Continental also threatened to sue United, the largest United States airline, over templates at Denver International Airport.
This method is a novel approach over template-wetting processes to fabricate assorted nanostructures.
Apple's concern over template-based apps for restaurants, Webb added, also doesn't make sense because "there's only so much you can do with apps that perform the same utility – ordering food".
Some young people dare to leave their stamp, sprayed in acrylics over templates made of cardboard, or as symbols painted on a column.
Additionally, Pol δ may also slide over templates containing direct repeats spaced by three or more base pairs, resulting in deletions of the nucleotides between these direct repeats.
PfuX7 allows the combined use of DpnI-treatment and dU-containing DNA to avoid carry-over of template.
TD of short sequences can be caused by unequal crossing-over or template slippage during DNA repair, producing tandem arrays (TARs) of homologous genes in close genomic vicinity (Kane et al. 2010).
Obviously, over-amplified templates reduce the accuracy of the statistical analysis, and the sequence reads derived from those templates must be filtered out as PCR bias before statistical analyses.
This "rare transcript" status can result from target mRNA being either endogenously rare by nature, made rare by experimental treatment or disease, by sheer sample degradation, or by over-diluting template-containing RNA or cDNA samples during qPCR set ups.
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