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At least three different exogenous genes could be expressed simultaneously and stably over multiple passages.
Phenotypic characterization of isolated hSMECs confirmed that a myoepithelial cell phenotype consistent with that from other exocrine tissues was maintained over multiple passages of culture.
The amniotic fluid (AF) is an invaluable source of organ-specific progenitor cells and stem cells that are easily obtainable, expandable over multiple passages, and can be used in various regenerative medicine applications.
We next determined whether differential expression of genes identified by microarray was maintained over multiple passages.
All isolated clones were viable and proliferated over multiple passages in classical culture conditions.
The heterogeneity in MCF7S seems to be rigidly maintained, as the proportion of self-renewing cells remains almost constant over multiple passages and after antiestrogen treatment (Figure 6B).
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Statistical analysis of the results for cell growth over single and multiple passages shows up to a 3-fold improvement in the consistency of cell growth kinetics with automated passaging.
It seemed justified that if these cells were to survive and propagate in culture for multiple passages (over 30), high levels of LC3 II (signifying abnormal autophagy) could be detrimental for the cells.
However, the replication of distinct prion conformers can be maintained in vivo over multiple serial passages which has not been shown so far for Aβ.
For trypsin incubation times greater than 10 min, especially over multiple ESC passages, a more dramatic decrease in the final viable cell number (∼80%) and Oct-4-GFP expression (∼70%) was measured.
Additionally, lines were karyotypically normal after multiple passages and have been propagated for over 30 passages in culture while retaining a normal karyotype (Figure S3).
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