Most of the delimitation methods recover 11 distinct evolutionary lineages, but molecular delimitation analyses, particularly those using only one line of evidence (in this case, mtDNA), can be prone to over-delimitation [ 90].
Problems have arisen over the delimitation of the various maritime zones between adjacent and opposing states.
Although China is a signatory to treaty, it has exercised its right (under Article 298) to absolve itself of any compulsory arbitration (under Article 287 and Annex VII) over territorial delimitation issues, among other things.
Both markers can vary substantially within fig wasp (and other) species, which may hinder correct species delimitation, in particular via over-splitting [ 37, 38].
While the sorting of mtDNA variation may, on average, track lineage divergence more rapidly than nDNA [14], there are important reasons for not relying on mtDNA as a sole source of evidence in species delimitation, including the potential to over diagnose mtDNA lineages influenced by selection [15] or sex-biased dispersal [57].
As the Court had not been mandated to draw the line of delimitation with respect to the territorial waters of Malaysia and Singapore in the area in question, it simply held that sovereignty over South Ledge belonged to the state which owned the territorial waters in which it is located.
Finally, phylogenetic error can diminish the accuracy of delimitation results.
In addition, the use of multiple loci in species delimitation has increased rapidly over the last few years and are currently being applied to a large number of taxonomic problems [ 2– 6].
Thus, this fauna provides an excellent opportunity to test the efficacy of barcoded-based species delimitation and identification of freshwater fishes over a broad geographic range.
GMYC is a species delimitation method that provides an objective way to delimit genetic clusters [ 56].
In spite of the numerous morphological characters available for species delimitation, the systematics of Hydrodictyaceae has been frequently debated and revised over the last decade [ 11– 14], with DNA sequence data revealing extensive cryptic diversity.
