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After 24 h, medium was change to EBM2 supplemented medium (Lonza) with 2% FBS (foetal bovine serum, Sigma-Aldrich) and 1×105 mBM-VPC from Dll4+/−, Dll4GOF and respective WT, were put over cultured HUVEC monolayer.
As transformed cells, the PC12 line has an advantage over cultured primary neurons, which do not maintain cell division and thus cannot detect adverse effects on the cell cycle, a likely neurotoxic target.
As transformed cells, the PC12 line has an advantage over cultured primary neurons, which do not maintain cell division and thus cannot detect adverse effects on the cell cycle, a likely neurotoxic target; furthermore, primary neurons do not provide a uniform population either in terms of cell types or differentiation state, rendering their use for screening problematic.
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Cells in these microcapsules exhibited improved cellular functions over those cultured on collagen monolayers.
The osteoblasts were able to migrate and proliferate over the cultured time for both PCL as well as PCL HA scaffolds.
The cells harvested from this system demonstrate improved attachment, morphology and functions over conventionally cultured cells, upon binding to ligand-conjugated polymer surfaces.
This was due to their hereditary service (instead of solely merit-based) and Confucian values that dictated those who chose the profession of violence (wu) over the cultured pursuits of knowledge (wen).
Transwells were placed over the cultured MDSCs, and HUVECs were added in basal RPMI at 1×105/well and allowed to migrate for 3.5 hours.
Endometrial stromal cells co-cultured with macrophage cell supernatant demonstrated significantly increased expression of CTGF over those cultured with vehicle.
Moreover, a curated set of over 1000 cultured bacterial and archaeal species from the human intestinal ecosystem has recently been reported [ 27].
A critical scientific and clinical issue is over whether cultured erythroid cells can be matured in vitro to generate enucleated erythrocytes.
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