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After being incubated for 24 h at room temperature while being vigorously mixed, the reaction mixture was lyophilized, dissolved in triethanolamine (TEA)/acetate buffer (pH 7.0), and immediately injected over a Luna analytical C18 RP-HPLC column (Phenomenex) with a gradient of 20 to 70% CH3CN/(TEA/acetate) over 60 min at a flow rate of 1 mL/min.
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In 2002, with Mr. Kongsgaard gone to focus on his own wines, Mr. Schoener took over at Luna. "It was a radical choice — he was a real freshman," said Mr. Kongsgaard, who has remained Mr. Schoener's mentor.
When it was over, Mr. Luna, 34, grinned and ran his hand through his wet hair.
The wood oven that put Luna on the map is still smoking away, and roasted chicken, a Luna signature, is still on the menu.
The camera cuts to Sisto, who deadpans: "sex with dead people?" and looks over at Luna.
The column was a LUNA C18 100A 150 × 2 mm (Phenomenex, Aschaffenburg, Germany).
We employed a Luna C8 5 μm (stainless steel, 150 × 4.6 mm i.d).
CLs were analyzed using a Luna C8 column (2.0 mm (i.d).
A Luna 5 μ C18 column, 250 × 4.6 mm (Phenomenex, USA) was used.
LC separation was achieved with a Luna reverse-phase C5 column (Phenomenex).
HPLC separation in LC-MS experiments was carried out on a Luna 5μm C18 (2) 100Å (250 × 4.60 mm I.D).
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