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Bathymetric relief on carbonate platforms was very gentle; the full spectrum of available communities was likely often expressed over a gradient hundreds of kilometres long.
For each country, 7 10 study fields were sampled over a gradient of increasing proportion of semi-natural habitats in the surrounding landscape.
We randomly selected and installed 52 plots over a gradient of MPB and fire severity combinations and measured stand structure and fire severity characteristics.
The peptides were then eluted over a gradient (0%45%% B in 55 min, 45%100%% B in 10 min, where B = 80% Acetonitrile, 0.1% formic acid) at a flow rate of 500 nL/min and introduced online into the linear ion trap mass spectrometer (ThermoFisher Corporation, San Jose, CA) using nano electrospray ionization (ESI).
The Starting buffer used was 50 mM sodium acetate buffer pH 4.0 with 1 M (NH4 2SO4 and proteins were eluted over a gradient of 0 to 58% (40 mL) and from 58 to 100% (30 mL) sodium acetate buffer pH 4.0.
The bound protein was then eluted over a gradient of 1 M imidazole (pH 8).
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A second and more comprehensive approach was proposed by Damgaard & Fayolle (2010) for quantifying the strength of competition in a pair of plant species as they changed over an environmental gradient (herbicide gradient).
A TUNEL assay showed fragmented nDNA in a gradient over these mitochondrial stages.
The product was purified by chromatography over alumina eluting with a gradient of CH2Cl2 to 10%CH3OH/90%CH2Cl2l2.
Here, we investigated processes behind beta diversity using ROV video, coupled with oceanographic and quantitative terrain parameters, over a depth gradient in Annan Seamount, Equatorial Atlantic.
The relationships among genetic, physiological, and ecological population-level responses of kelp forests occurred over a temperature gradient of similar magnitude to projected sea temperature increases in the upcoming 50 100 years10.
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