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Data were obtained using the isothermal oven procedure and analyzed using the Frank Kamenetskii method and a scaling procedure, both contemplated in standard EN15188.
Constrained simplex-centroid design was applied in the optimization of the digestion in microwave oven procedure, and the results evaluated from topological maps of the Kohonen network.
The microwave oven procedure was optimized to obtain extraction efficiencies similar to the conventional BCR procedure, in less time, while using smaller volumes of reagents.
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For hybridization, 1.65 µg of Cy3-labeled cRNA was added on microarray slide for 17 hours using the Hybridization Oven kit procedure provided by Agilent Technologies.
Sections were deparaffinized with xylene and rehydrated in graded ethyl alcohol, sections were immersed in citrate buffer solution of pH 4.8 and were put in the microwave oven before staining procedures.
Milk samples can be efficiently digested using a focused microwave oven, however the conventional procedure of addition of concentrated acids to the liquid sample leads to digestates with elevated acidity and residual carbon concentrations.
The mass loss of composting mixture at termination of experiment was calculated by gravimetric procedure on oven-dry weight basis (Frederick et al. 2004).
The oven and sun drying procedures were employed where temperatures were maintained between 50 and 70 °C (oven drying) and sun drying, the readings were observed at every one hour.
Five μg total RNA from each sample was used in the regular protocols for GeneChip® Arrays and hybridized onto the Lund-zfa Affymetrix array overnight in the GeneChip® Hybridisation oven 6400 using standard procedures.
The hybridization reaction was performed at 65°C for 17 hours using the Agilent DNA microarray hybridization oven (Agilent Technologies), following procedures described in the Agilent One-Color Microarray-Based Gene Expression Analysis protocol.
This was used as a template to generate biotin-targeted cRNA following manufacturer's specifications; 15 μg of the biotin-labeled cRNA was fragmented to strands between 35 and 200 bases in length, 10 μg of which was hybridized onto the GeneChip Human Gene 1.0 ST whole transcript based assay overnight in the GeneChip Hybridization oven 6400 using standard procedures.
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