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Nonetheless, the outward modifications were clearly distinguishable from the normal color-pattern (Fig. 4H-J).
Most tenth generation cold-shocked individuals showed extensive outward modifications (Fig. 4E-G).
Indeed, the inward and outward modifications never coexisted in a single individual.
Eclosed virgin adults with relatively severe outward modifications from each group were selected and kept in a cage for mating, and the resulting eggs were collected.
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However, modified individuals have been occasionally reported in the Japanese lepidopterological journals including the outward-type modifications (e.g., [ 16]) as well as those of the inward and reduction types (e.g., [ 17, 18]) from many localities.
From the sixth to tenth generations without immediate treatment to pupae, the proportion of the outward type (defined as modification rate, MR) suddenly increased to 30-50% and appeared to be further increasing up until the tenth generation, although the degree of modifications in a given individual was not very severe even in the tenth generation.
Although we do not know whether the modification of gemcitabine affects the outward conformation due to the lack of a structure of the outward-facing conformation, our studies suggest that destabilization of the inward-facing-occluded step would facilitate the release of the pyrrolo-gemcitabine.
We found that cholesterol depletion caused a selective modification of ion channel function, with whole cell inward Ca2+ current being unaltered, whereas outward K+ current was increased, due to BKCa channel activation.
The increase of the outward type in males from 2002 to 2004 in Fukaura likely indicates ongoing process of genetic assimilation of this modification trait in the population.
Outward Bound.
Outward appearances are important.
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