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The same phenomenon was reported previously [ 31] when K+ secretion was first diminished by raising the apical K+ concentration, thereby reducing the outward gradient across the apical cell membrane.
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When Λ < 0 (outward pressure gradient) both branches are stable.
We cannot exclude that glucose loading to create an outward facing gradient could induce a trans-stimulation effect.
This view is further supported by the result that an outward proton gradient from intracellular fluid to extracellular medium effectively enhanced diphenhydramine uptake by hCMEC/D3 cells.
In GLUT3 transfected cells, a significant increase in glucose transport rate (above that attributable to transport via endogenously expressed GLUT12) was measured under conditions designed to create an inward but not outward glucose gradient.
The water content was nearly uniform throughout the BC, whereas an outward-decreasing gradient was observed in the BFM.
Further, like in the findings of Mphinyane (2001) in which Acacia gerrardii's density increased with distance away from the piosphere (cattle post), several woody species (e.g. Acacia drepanolobium, Acacia xanthopholea, Maerua pseudopetalosa and Triumfetta annua) were found to increase outward with the gradient distance.
Subsequently a membrane potential was generated by either an inward or outward directed potassium gradient.
Anion exchange activity was measured as the change (elevation) in pHi because of HCO3− ion exchanged into the intracellular compartment driven by the Cl− ion concentration gradient (outward export).
In combination our experiments show that under conditions designed to create an outward facing glucose gradient, glucose transport is pH dependent, is inhibited by cytochalasin B, requires the presence of an inward facing electrochemical gradient and results in intra-cellular acidification.
Glucose transport assays were then initiated by the addition of 2× uptake solution (either pH 7.4 or 5.0) containing 5 mM cold 2-D-deoxyglucose (to create an outward facing hexose gradient with a final total extracellular hexose concentration of 5 mM) and 1 μCi/ml 2-[1,2-3H(N ]deoxy-d-glucose.
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