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Thus, Ku70 α5 is utilized for both heterotetramerization, where the two outward faces in each heterodimer interact with each other, and NHEJ, suggesting NHEJ requires Ku heterotetramerization.
Together, our data lead us to propose a model in which Ku heterotetramerization occurs via an interaction of the two outward faces of each Ku heterodimer with Ku70 α5 at the interface.
Thus, the findings that coexpressed N-terminally tagged Ku70 proteins were able to reconstitute fluorescence in the PCA but coexpressed N-terminally tagged Ku80 could not are consistent with Ku heterodimers interacting with each other in an orientation bringing the outward faces together.
Because interactions involving the outward faces of two Ku heterodimers might contribute to the synapsis of the two ends of a DSB during NHEJ, we then asked whether Ku70 α5 might contribute to Ku heterotetramerization (i.e., the association of two Ku heterodimers) and thereby facilitate NHEJ.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com