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We tested the sensitivity of the outward current to the GABAA receptor antagonist, bicuculline (30 µM).
The best-known flow of K+ is the outward current following depolarization of the membrane.
Given the surprising result of a glutamate transporter mediating the increase of an outward current in dopamine neurons, we next determined whether the outward current was indeed an IPSC mediated by an inhibitory input.
Comparison of maximum inward and outward current densities, normalized to cell capacitance (Supplementary Fig. 5c), revealed significant genotype differences in inward current density as well as sustained and transient outward current densities (Supplementary Fig. 5d f).
Supplementary Figure 5a depicts example inward and outward current traces from a control neuron at week 12 in vitro.
Experimental observations of variable AP morphology could be accounted for by changes in transient outward current density, as suggested experimentally.
After Leishmania infection, macrophage activation is characterised by a reduction of nitric oxide production and of outward current density.
Under conditions designed to isolate H+ currents, a voltage-dependent outward current was recorded from whole-cell patch-clamped neurons.
Paxilline (500 nM -sensitive componM -sensitiveard componentsre significantly decreased in bofh irradiated and gene silencing SMCs.
The validity of this approach is supported by the observation that 100 μM picrotoxin completely eliminated all spontaneous outward current activity recorded at 0 mV (not shown).
Three outward potassium currents have been found in LCs [28], [29]: an early outward current IA, a delayed outward current IKd, and a calcium-dependent potassium current IKCa.
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