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Outside, patterns emerged dependent on the shuttle schedule.
The "inside and outside" patterns in the following results were determined using these two indexes.
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The "inside and outside" pattern is important because this pattern reflects an original embryonic structure [ 1].
We explored various parameter sets with which the "inside and outside" pattern could be achieved.
On the other hand, the DAH model can also explain the "inside and outside" pattern.
Although the "inside and outside" pattern is important, as it is reminiscent of the original embryonic structures, the SAH model could not reasonably explain the "inside and outside" pattern as described in the Background section [ 9].
Although early versions of the DAH model could not reproduce the "inside and outside" pattern, the Glaizer's model, at last, reproduced the "inside and outside" pattern excellently based on the DAH [ 23, 24].
The model could predict directional cell movement and its mobility are important in the "inside and outside" pattern of cell sorting.
Our present model overcomes this weakness in the SAH because it successfully recapitulated the "inside and outside" pattern based on the SAH.
When GAP PREFERENCE of both cell types was set at Htr, the "inside and outside" pattern of cell sorting was reproducibly attained using certain parameter sets (Fig. 6c,d); however, an "inside and outside" configuration was not obtained when the GAP PREFERENCE of both cell types was set at Hm (Fig. 6g l).
Based on these observations and consideration, we constructed an in silico cell model with a flexible shape, directional motility, and sensitivity to parameters like autonomous cellular behaviors; we then used this model to examine what differences in cellular behavior parameters would lead to the reproducible generation of an "inside and outside" pattern of cell-sorting.
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