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That this fluorescence change is seen even with the R270K enzyme provides further evidence that phenylalanine activation is due to binding outside of the active site.
Therefore, it appears that TYR71 residue keeps the direction pointing outside of the active site.
A systematic deletion study, which again assayed cleavage qualitatively, indicated that the activity also depends on the lysine triad that is near but outside of the active site.
The studies indicate an important role of amino acid residues outside of the active site, which would be difficult to target by other methods.
There was a sudden drop of induced charge at the trench but a long tail was present outside of the active area extending several tens of microns.
However, Na+ and Li+ reduce the magnitude of the allosteric response to Fru-1,6-BP, likely due to mechanisms outside of the active site.
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This report identifies a critical residue in the TPPI sequence that is outside of the active-site triad and is still essential for the enzyme activity.
The percentage of Gd-DTPA enhancing volume outside the area of the active tumour volume at a MET uptake threshold of ≥1.3 is shown for each patient in Table 4.
In addition, because other sites of both activating mutations and inhibitor binding can occur well outside of the Abl active site, there seems to be evidence supporting the existence of long-range conformational coupling within the Abl kinase core.
The reconstructed AncMDH2 and AncLDH sequences have 31 and 48 ambiguous positions, respectively, all of which are located outside of the 'first active site shell' (defined as within 6 Å of the substrate).
Just outside the mat, the composition of the active archaeal community shifts to nearly exclusively Marine Group I at the sediment surface, and ANME-2a at 12 15 cmbsf (Fig. 4a).
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