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In the current design, the saturated water flows into a cylindrical chamber with a tube array, whereas the hot air travels outside of chamber and boils the water inside.
The apparatus consists of a chamber A containing a smaller sized chamber B having copper wire mesh sheet on top and connected by a side tunnel to chamber C outside of chamber A. Chamber A was used to place the test sample, and chamber B was used to place the mosquitoes.
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For the migration assays, 5 × 10 cells suspended in 400 μl of DMEM containing 10% FBS were seeded onto the upper chamber, whereas 600 μl of DMEM containing 10% FBS was added to the outside of the chamber.
In total, 1 × 10 cells in 400 μl of DMEM containing 10% FBS were seeded onto the upper chamber, whereas 600 μl of DMEM containing 10% FBS was added to the outside of the chamber.
The opening of the diaphragm is controlled from the outside of the chamber by means of the magnetic system.
The W needle can be linearly moved from outside of the chamber, and therefore we can easily vary the distance between the W needle and anode plate.
The 8 × 8 cm odor sampling port was mounted on the outside of the chamber and an 8 cm diameter hole cut through the back wall and sampling port allowed access for the animal's snout.
Fasten the wire you let hang from the grill ignitor to this screw on the outside of the chamber.
The procollagen that entered the chambers via the pores, was assumed to have been produced by the packed layer of peritoneal macrophages that adhered firmly to the outside of washed chambers.
Despite their standing as the intellectual lions of the left and right, Ginsburg and Scalia have forged an uncommon bond on a court where close friendships outside of chambers are rare.
A week later, I'm hearing random people in my just-outside-of-the-echo-chamber coffee spot talk about it like they'd talk about the weather.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com