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CF = Centre forward FB = Fullback HB = Defender GK = Goalkeeper IF = Inside forward OF = Outside forward.
The sequences of mutagenic oligonucleotides are listed in Table 3, and the outside forward oDYR07-522) and reverse primers oDYR07-322) were previously described [11].
Nested PCR amplification on converted DNA used the following primers: outside forward: 5'-GTGTGGGATTTAGGTTTTTAAG-3' (SB1); outside reverse:5'-CCAAATCCAACCTCATAATATATC-3' (SB2); and nested inner primers (SB3) and (SB4) whose sequences and locations are described in detail below.
The following nested sets of converted PCR primer oligonucleotides used to amplify this chromosome 2 region were synthesized by Sigma: outside forward primer, 5'-TGGAGTAGAAAATATGGTT AGGAATATTGG-3'; outside reverse primer, 5'-CACTCACAACATCTAAAACTACAAA CAACC-3'; inside forward primer, 5'-TGGTGTTTTAGATGATGGTAGTGGG-3', and inside reverse primer, 5'-ACAATAACATTCCTTCCTCCAC-3'.
Caveolin-1 MSP-specific PCR primers were designed as shown in [ 8, 9]: outside forward 5′-AGGATAGGGTAGGATTGTGG-3′ and outside reverse 5′-CATAAA ACATTCCTAACTTCTCTTCACCTC-3′; methylated forward 5′-GGTATTTTTGTAGGCGCGTC-3′ and methylated reverse 5′-CTAACAACAAAAAACGAAAAACG-3′; unmethylated forward 5′-GTTTATATTGGGTATTTTTGTAGGTGTGT-3′ and unmethylated reverse 5′-TCCCCAAAATTCTAACAACAAAAAACAAAAAAC-3′.
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The team played in a 2 3 5 formation throughout the campaign, with two fullbacks, three halfbacks, two outside forwards, two inside forwards and a centre forward.
The team played in a 2 3 5 formation (the standard formation at the time) throughout the campaign, with two fullbacks, three halfbacks, two outside forwards, two inside forwards and a centre forward.
The team played in a 2 3 5 formation (the standard formation at the time) throughout the campaign, with two full-backs, three half-backs, two outside forwards, two inside forwards and a centre forward.
Randle is a 6-foot-9 inside-outside forward who can overpower smaller players and outpace other bigs.
Outside-forward Ted Vizard played the ball into a central position and Jack Smith hit the ball past Hufton.
The outside-forward then cut inside and played the ball across goal in a manner described by some correspondents as a shot and others as a pass.
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